NASA scientists are sending three fundamental life science
experiments onboard space shuttle Discovery in hopes of better
understanding exactly how spaceflight affects cell growth and how cells
fight off infections. Future astronauts on long-term space missions need
to understand how wounds heal and cells become infected in space to
prevent illnesses during space travel.
When space shuttle
Discovery
hurtles into orbit after its scheduled launch on 5th April, in addition
to the multi-purpose logistics module filled with science racks for the
laboratories aboard the station it will carry seven astronauts, two
Space Tissue Loss experiments and 16 mice as it rendezvous with the
International Space Station.
“As we expand humanity’s reach to other planets we must learn how to
live in space for prolonged periods of time,” said Eduardo Almeida, the
Space Tissue Loss’s Stem Cell Regeneration experiment principal
investigator and scientist at NASA’s Ames Research Center in Calironia.
“Understanding how space affects stem cell health is critical to
exploration because our health relies on normal tissue repair and
regenerative functions.”
Stem Cell Regeneration experiment will study how embryonic stem cells
develop into specialized tissue types, or “differentiate” in space. The
experiment will use mouse embryonic stem cells and embryoid bodies, or
ball-shaped collections of embryonic stem cells, as a model to study the
effects of microgravity on adult stem cells’ ability to carry out their
normal function of repairing and regenerating tissues. Scientists
compare the embryoid body to an early stage of development in mammals
because embryonic stem cells can differentiate into any of the body’s
many cell types.
In the weeks leading up to launch, scientists working on the Stem
Cell Regeneration experiment at NASA’s Kennedy Space Center, Florida,
grew mouse embryonic stem cells and prepared them for flight. Scientists
will take the embryonic stems cells grown in the laboratory and place
them into bioreactors, which are installed into an incubator that fits
into a shuttle middeck locker, where they will remain during flight.
“We are trying to get at the root cause of tissue degeneration in
space,” said Almeida. “We hope our research will help find preventive
measures to address adult stem cell health in microgravity.”
The second Space Tissue Loss (STL) experiment, STL-Immune, led by
principal investigator Cheryl Nickerson, associate professor of life
sciences at the Center for Infectious Diseases and Vaccinology in the
Biodesign Institute at Arizona State University, will be the first
fundamental biology experiment to conduct an in-flight infection of
human cells using pathogenic bacteria. Nickerson’s experiment will
characterize the effect of microgravity on intestinal cellular responses
before and after Salmonella infection during space flight.
“In addition, this experiment also closely monitors human cells
giving us unique insight into conditions faced by astronauts during
spaceflight, as well as how cells in our bodies normally behave or
transition to disease caused by infection, immune disorders or cancer,”
said Nickerson. “Only by studying how cells respond to microgravity can
we reveal important biological characteristics that are masked by normal
gravity when using traditional experimental approaches on Earth.”
The
Immune experiment will help scientists determine whether bacterial
responses to spaceflight are also seen in human cells.
“Better understanding how microbes and human cells interact in space
can lead to novel vaccines and therapeutics for the general public
against infectious disease, as well as other human diseases,” added
Nickerson. “Our research has potential benefits and applications for
life on Earth and astronauts on long-duration space missions.”
Mouse Immunology, the third space-based experiment, will study the
influence of microgravity on mice immune systems. The experiment’s
principal investigator, Millie Hughes-Fulford, former NASA astronaut and
professor in the Departments of Medicine and Urology at the University
of California, San Francisco will test whether an immune system response
to a new infection or re-infection is affected by spaceflight.
“Mouse immunology will allow us to pinpoint which genes and pathways
are or aren’t working or performing well in space,” said Hughes-Fulford.
“We will examine all 8,000 genes of the mouse thymus cell to determine
the molecular cause of a suppressed immune system.”
Before launch, half of the mice in both the group that will fly to
space and the control group that will stay on Earth received white blood
cells that had been inoculated with thymus cells, or white blood cells,
that were exposed to a foreign protein challenge. The other half of the
mice will not be exposed until immediately after they return from
space. Scientists will analyze whether the mice that received white
blood cells react differently than those that were not pre-exposed.
All three experiments are managed by the International Space Station
Non-Exploration Projects Office at NASA Ames. The NASA Ames Flight
Systems Implementation Branch and Space Biosciences Division developed
and implemented the Mouse Immunology and Space Tissue Loss payloads,
which were all funded by the Advanced Capabilities Division in the
Exploration Systems Mission Directorate at NASA’s Headquarters,
Washington.
The Walter Reed Army Institute of Research based in Silver Spring,
Maryland, provided the hardware and the Department of Defense’s Space
Test Program developed the payload and managed the hardware integration
for the Space Tissue Loss Experiments.
• For more information about science on the International Space Station, visit: www.nasa.gov/mission_pages/station/science
• For more information about the Space Biosciences Division at NASA Ames, visit: http://spacebiosciences.arc.nasa.gov